Clinical and Laboratory Studies on Dogs Suffering from Pyoderma in Correlation to some Microelements and Some Oxidative Stress Markers

The present study was carried out in the clinic of Faculties of Veterinary Medicine Suez Canal University, Ismailia and Cairo University, Cairo, Egypt on household dogs and cats admitted to both clinics and suffer from skin problems. The study was continued for one year. The clinical study was conducted on two groups of dogs. The first one was the diseased included ninety (90) dogs of different ages. The second group was clinically healthy fourteen (14) dogs used as a control group. Animals were examined clinically and special dermatological examination. Skin scraping and ear swapping samples were examined under microscope. Blood samples were taken for complete blood pictures and separation of plasma. Plasma used for analysis of zinc, Cu and oxidative stress markers including catalase, SOD and GPx. The history of diseased dogs suffered from pyoderma indicated that they were suffered from a previous mite, fungal infection, external parasitic infestation or a previous allergy signs. Clinical examination revealed that 17 out of ninety skin diseased dogs were diagnosed as Pyoderma with a ratio of 18.9 %. The most common lesions were erythema, papules, pustules, erosions, alopecia and Pruritus. The hematological analysis of pyoderma affected dogs showed anemia due to the highly significant decrease in hemoglobin value and MCHC value. A significant increase in total leukocytic count and lymphocyte % and a highly significant increase in granulocyte and monocytes % were prevalent. A significant increase in both catalase activity and SOD values in diseased dogs. A high positive correlation between plasma Zn and plasma Cu levels, with high negative correlation between plasma Zn level and plasma catalase activity. Very high negative correlation between plasma catalase activity and plasma MDA level was recorded with Moderate negative correlation between plasma SOD activity and plasma MDA level and a moderate negative correlation between plasma MDA level and plasma GPX level. The previous correlations of minerals levels, enzymes activities and the acute phase proteins could be used as biomarkers for pyoderma Keywords: Dog pyoderma, correlation, catalase, clinical signs